Angiogenesis and scar inhibition after subcutaneous implantation of Shengji Yuhong collagen

doi:10.3969/j.issn.2095-4344.2013.34.013

Abstract

Abstract:

BACKGROUND: Shengji Yuhong collagen showed good curative effect of promoting angiogenesis and tissue healing compared with Shengji Yuhong Gao and collagen alone or gelatin alone.

OBJECTIVE: To explore the curative effect and mechanism of subcutaneous implantation of Shengji Yuhong collagen in rabbits in promoting angiogenesis and repair.

METHODS:Shengji Yuhong collagen as the experimental group and collagen as the control group was implanted inside the rabbit subcutaneous pockets of the back of New Zealand rabbits. The implanted samples and surrounding tissues were obtained at 3, 7, 14, 28 and 56 days following surgery. Pathological sections were made and the repair of surrounding tissue was observed. Hemoglobin levels in collagen were measured. Immunofluorescence and CD34 dyeing marking method were utilized to observe capillary angiogenesis. Western blot assay was employed to examine vascular endothelial growth factor and angiogenin-1 expression. Immunohistochemistry was used to observe the secretion of typeⅠ and Ⅲ collagen on the surrounding tissues.

RESULTS AND CONCLUSION: The experimental group showed increased subcutaneous vascularization. There were reduced inflammatory exudation, granulation tissue hyperplasia, and mature fiber connective tissue at 28 days. Angiogenesis and hemoglobin contents were greater in the experimental group than in the control group (P < 0.05 or P < 0.01). At 3 and 7 days following surgery, vascular endothelial growth factor and angiogenin-1 expression was greater in the experimental group than that in the control group (P < 0.05 or P < 0.01). The secretion of type Ⅰ collagen was identical between the experimental and control groups. However, the secretion of type Ⅲ collagen was higher in the experimental group than in the control group at 28 and 56 days (P < 0.05), and the proportion of type Ⅰ and Ⅲ collagen was lower in the experimental group than in the control group at 28 and 56 days (P < 0.01). These suggested that Shengji Yuhong collagen can significantly promote angiogenesis in the surrounding tissues with the possible mechanisms of adjusting the protein expression of vascular endothelial growth factor and angiogenin-1. At the same time, it has the function of regulating collagen formation with better ratio of typeⅠ and type Ⅲ collagen to acquire higher quality of wound healing with reduced scar formation.

Key words: biomaterials, material biocompatibility, Shengji Yuhong collagen, angiogenesis, typeⅠcollagen, type Ⅲ collagen, vascular endothelial growth factor, angiogenin-1, National Natural Science Foundation of China

CLC Number:

R318

Cao Dong-yang, Yao Chang, Chen De-xuan, Bian Wei-he, Zhang Xiao-qing, Yin Heng, Guo Meng-meng. Angiogenesis and scar inhibition after subcutaneous implantation of Shengji Yuhong collagen[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(34): 6144-6151.

Figures/Tables 9

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